alexa Simultaneous Estimation of Antihypertensive and Antidiabetic Drugs by HPLC

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Research Article

Simultaneous Estimation of Antihypertensive and Antidiabetic Drugs by HPLC

Mohammad Farhadur Rahman1, Md. Zakir Sultan2*, Asma Rahman2, Md. Anisur Rahman1 and Md.bdus Salam1

1Department of Chemistry, University of Dhaka, Dhaka-1000, Bangladesh

2Centre for Advanced Research in Sciences, University of Dhaka, Dhaka-1000, Bangladesh

*Corresponding Author:
Md. Zakir Sultan
Centre for Advanced Research in Sciences
University of Dhaka, Dhaka- 000, Bangladesh
Tel: +88-02-9661900-73 Extn: (4722)
Fax: +88-02-9667222
E-mail: [email protected]

Received date:14 June 2015; Accepted date: 28 July 2015; Published date: 04 August 2015

 

Abstract

A simple, fast and economic reversed phase high performance liquid chromatographic (HPLC) method has been successfully developed and validated for simultaneous determination of an antihypertensive and an antidiabetic drug namely, valsartan and glimepiride in pure form (as API). Before the method development with HPLC, a complexation study was carried out with these drugs by DSC which demonstrated no complexation among these drugs. Therefore, a simple HPLC method was developed and validated according to the guidelines of ICH, FDA and USP with respect to accuracy, precision and linearity. For method development a C-18 bonded silica column (250 x 4.6 mm, 5μ, Phenomenex, Inc.) and the mobile phase comprising 3% aqueous acetic acid in acetonitrile (60:40, v/v) at a flow rate of 0.7 ml/min were used. For accuracy, the recovery was found to be more than 99% for each of valsartan and glimepiride. Intra-day and inter-day precisions of the new method were less than the maximum allowable limit (RSD% 2.0) according to FDA guidelines. The method showed linear response with correlation coefficient of 0.9993 for valsartan and 0.9981 for glimepiride. Therefore, the developed method was found to be simpler, accurate, reproducible, efficient and less time consuming and can be successfully applied for the simultaneous estimation of valsartan and glimepiride.

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