alexa Spectrofluorometric and UV Spectrophotometric Methods for the Determination of Flurbiprofen in Pharmaceutical Preparations
e-ISSN: 2320-0812 p-ISSN: 2347-2340

Research & Reviews: Journal of Pharmaceutical Analysis
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Research Article

Spectrofluorometric and UV Spectrophotometric Methods for the Determination of Flurbiprofen in Pharmaceutical Preparations

Bilal Yilmaz*, Emrah Alkan

Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, 25240, Erzurum, Turkey

*Corresponding Author:
Bilal Yilmaz
Department of Analytical Chemistry, Faculty of Pharmacy
Ataturk University, 25240, Erzurum, Turkey
Tel: +90 4422315200
Fax: +90 4422315201
E-mail: [email protected]

Received date: 20/10/2015; Accepted date: 02/11/2015; Published date: 10/11/2015



In this study, a new and rapid spectrofluorometry and UV spectrophotometry methods were developed for determination of flurbiprofen in pure and pharmaceutical preparations. The solvent system and wavelength of detection were optimized in order to maximize the sensitivity of both the proposed methods. The linearity was established over the concentration range of 50- 350 ng ml-1 for spectrofluorometry and 1-14 μg ml-1 for UV spectrophotometry method. The intra- and inter-day relative standard deviation (RSD) was less than 3.80 and 3.20% for spectrofluorometry and UV spectrophotometry, respectively. Limits of quantification (LOQ) were determined as 0.03 and 0.60 μg ml-1 for spectrofluorometry and UV spectrophotometry, respectively. No interference was found from tablet excipients at the selected assay conditions. Also, the methods were applied for the quality control of five commercial flurbiprofen dosage forms to quantify the drug and to check the formulation content uniformity.


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