Studies on Humoral Immunity in Fresh Water Crab Barytelphusa Guerini
Crustaceans contain two types of enzyme catalyzing phenol oxidation; one being hemocyanin and the other being expressed from hemocytes as its precursor prophenoloxidase. In the present work prophenoloxidase and lysozyme activity was studied in Barytelphusa guerini. Prophenoloxidase gradually increased from 2hrs to 24 hrs and then started decreasing from 24hrs to 48hrs after challenged with gram positive and gram negative bacteria in case of male crabs. In case of female crabs challenged with bacteria, phenoloxidase activity gradually increased from 2hrs to 12 hrs and highest at 12hrs interval after challenge and started decreasing from 12hrs to 48 hrs of post bacterial inoculation. The enzyme activity in hemolymph of bacterial challenged crabs was highest in all time points than control and control injured crabs. Enzymatic activity gradually increased from 2hrs to 12 hrs and the decreased from 12hrs to 48hrs in case of challenged male crabs and female challenged crabs when challenged with E.coli and S.aureus. Highest enzymatic activity was observed at 12 hrs of post bacterial challenge. The highest zone of inhibition observed at 12 hrs in case of E.coli challenged crabs in male crab and female crabs. The zone of inhibition increase gradually from 2hrs to 12 hrs in E.coli challenged male crab and female crab and also in male and female crabs challenged with S.aureus. The protein band observed at 15 kDa was identified as Lysozyme and 66kDa as Prophenoloxidase.