Studies on Mycoflora Associated with Market Roots of Drug Solanum indicum Vent. and their Effect on Changes of Chemical Constituents under Storage
In the present study the market roots of drug plant Solanum indicum Linn. were selected for isolation of mycoflora associated with market roots and changes in chemical constituents amount under storage of different relative humidity and incubation days due to spoilage of fungal contamination. Isolation and identification of fungi and phytochemical analysis were done according standard methods. The results showed that total 12 fungi were associated with market root drug. A. niger showed highest percentage incidence while Stemphylium paxianum and Alternaria sp. observed in minimum percentage incidence. The drug stored under influence of different relative humidities viz. 30, 50, 75, 96 and 100% and different incubation days 15, 30, 45, 60 days. Quantitative estimation of chemical constituent’s amount such as sugars, proteins, phenols, alkaloids and glycosides in relation to association of fungi was done. The results indicated that maximum growth and percentage incidence of fungi as well as biodeterioration of chemical constituents observed under high relative humidities 75, 96 and 100% RH and long storage periods 45 and 60 days. Analysis of variance also showed that the effect of relative humidity and incubation days on biodeterioration of chemical constituents amount were significant.