|"One type of PTM peptides, phosphopeptide, is usually isolated from the total cellular peptide mixture using the immobilized metal-ion affinity chromatography(IMAC), and followed by mass spectrometry analysis to calculate quantitative changes at PTM level. Similarly, to enrich the proteins containing sulfhydryl groups in a MS-based thiol redox study, a thiol affinity chromatography has been developed . Further improvement on this type of method using the biotin-tagged thiol-reactive reagent has made it an acceptable protocol in the thiol redox proteomics. The MS-based thiol redox status measurement methods provide site- specific information in the redox state of cysteines as compared to the gel-based approach.
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Citation: Hu Q, Guo G, Yang Z, Li Y, Xia Y (2014) Stable Isotope Metabolic Labeling-Based Quantitative Thiol Redox Proteomic Analysis of Hydrogen Peroxide-treated Arabidopsis plant. J Proteomics Bioinform 7: 121-133."