Phanerochaete Chrysosporium

"Phanerochaete chrysosporium was investigated in shaken liquid cultures using the nutrient-rich maltextract broth medium, pH 4.5 containing malt extract (20 g/l), dextrose(20 g/l) and peptone (1 g/l). Tolerance to Cd was investigated in the0-2 mM concentration range whereas that to PAHs was tested in the 10-25 ppm range. For preparation of fungal inoculum, a 50 mlstationaryculture grown in a Fernbach flask for 48 hours at 37°C was homogenizedaseptically to prepare a uniform suspension as described previously. Liquid cultures (50 ml) werset up in 125 ml Erlenmeyer flasks by inoculating the culture medium (malt extract broth, pH 4.5) at 10% v/v.The cultures were grown for 24 hours by shaking (180 rpm) at 37°C. For assessing the effect of metal and/or PAH on the growth, calculatedamount of the test xenobiotic were spiked and the growth continued until 72 hours under the same incubation conditions. Mycelial biomasss harvested by vacuum filtration using Whatman No.1 filter paper and dried at 80°C for 24 hours or until a constant weight was achieved. OMICS Group International is one of the leading Open Access Publishers which is publishing 700+ peer-reviewed journals with the support of 50,000+ editorial board members as editorial team and aimed to disseminate the scholarly knowledge to the scientific society. OMICS Group also organizing 3000+ International Scientific Conferences and events yearly all over the world with the support of 1000+ Scientific associations worldwide.

• Share this page
• 
• 
• 
• 
• 
• 

Last date updated on April, 2024