"Phanerochaete chrysosporium was investigated in shaken liquid cultures using the nutrient-rich maltextract broth medium, pH 4.5 containing malt extract (20 g/l), dextrose(20 g/l) and peptone (1 g/l). Tolerance to Cd was investigated in the0-2 mM concentration range whereas that to PAHs was tested in the 10-25 ppm range. For preparation of fungal inoculum, a 50 mlstationaryculture grown in a Fernbach flask for 48 hours at 37Â°C was homogenizedaseptically to prepare a uniform suspension as described previously. Liquid cultures (50 ml) werset up in 125 ml Erlenmeyer flasks by inoculating the culture medium (malt extract broth, pH 4.5) at 10% v/v.The cultures were grown for 24 hours by shaking (180 rpm) at 37Â°C. For assessing the effect of metal and/or PAH on the growth, calculatedamount of the test xenobiotic were spiked and the growth continued until 72 hours under the same incubation conditions. Mycelial biomasss harvested by vacuum filtration using Whatman No.1 filter paper and dried at 80Â°C for 24 hours or until a constant weight was achieved.
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Last date updated on July, 2014