In two branches of poultry culture; small local ones and big industrial ones, tetracycline is a common antibiotic, which has been taken as a standard antibiotic in this study. After collection of samples, total numbers of bacteria with and without tetracycline were counted. In both cases numerous bacterial growths were observed. High concentration of antibiotics for example, above 100 µg/ml may be harmful to humans and animals. After performing sensitivity test against other commonly used antibiotics in poultry, it was found that isolated tetracycline-resistant E. coli were 100% resistant to penicillin and erythromycin,100 sensitive to imipenem, 93.34% resistant to tetracycline, 23.03% resistant to gentamycin and 53.33% resistant to chloramphenicol. These indicated the multidrug resistant property of isolates. Subsequent agarose gel electrophoresis showed no plasmid DNA band in the gel indicating non-existence of any bacterial plasmid and also proved that the observed resistance was chromosomal gene-mediated or at least not plasmid mediated. referred by : Abu Sayeed Mohammad Mahmud(https://www.omicsonline.org/open-access/isolation-and-identification-of-nonplasmid-multidrug-resistant-ecoli-from-poultry-wastes-in-chittagong-region-2155-9597.1000182.php?aid=23316)
Last date updated on May, 2024
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