"A NEP 2320 Barocycler (Pressure Biosciences) modified by the manufacturer to hold isobaric pressure was also used to develop pressure-assisted extraction protocols. Pressure cycling technology, consisting of repeated cycles of high pressure to low pressure, has been used to recover proteins, lipids and nucleic acids from fresh tissue. Our laboratory group investigated using pressure cycling to augment protein recovery from FFPE tissue surrogates, but determined that static pressure and temperature over 65Â°C were move effective in reversing formaldehyde cross-links.
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Citation: Fowler CB, OâLearyTJ, MasonJT (2014) Improving the ProteomicAnalysis of Archival Tissue by Using Pressure-Assisted Protein Extraction:A Mechanistic Approach.J Proteomics Bioinform7: 151-157."
Last date updated on July, 2014