The proliferation assay was set up on a restimulation day for the MBP-specific T cell cultures or on the day of plating for the primary culture. The assay was performed in 96-well plates (Corning, Wilmington, NC) with 8-9A104 MBP-specific T cells per well and a 6- to 10-fold excess of irradiated (3,000) isogenic speloncytes as antigen presenting cells per well.
OMICS Group International is one of the leading Open Access Publishers which is publishing 700+ peer-reviewed journals. Journal of Bacteriology & Parasitology is one of the top journals which publish quality articles, findings dealing with Bacteria and Parasites of Medical relevance to human or veterinary health.
Last date updated on January, 2021