Protein Lysate|Journals Open Access|Protein Lysate

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Protein Lysate

"Protein lysates of osteosarcoma cell lines were used in this study. The lysates were prepared in the same way as in our previous study. A lysate of HuO9 cells was used to examine the gel images of individual samples, and lysates of HuO9, HOS, MG-63, MNNG-HOS, 14 3B, HS-Os-1, NOS-1, and NOS-10 cells were used to create the common internal standard sample employed in this study. In brief, the monolayer cells were washed twice with ice-cold PBS, and fixed with 10% tri chloro acetic acid for 30 min. The cells were then scraped off, and washed with PBS. They were then treated with protein lysis buffer (2M thiourea, 6M urea, 3% CHAPS, 1% Triton X-100) for 30 min. After centrifugation at 15,000 rpm for 30 min, the supernatant was recovered as a protein lysate. OMICS Group International is one of the leading Open Access Publishers which is publishing 700+ peer-reviewed journals with the support of 50,000+ editorial board members as editorial team and aimed to disseminate the scholarly knowledge to the scientific society. OMICS Group also organizing 3000+ International Scientific Conferences and events yearly all over the world with the support of 1000+ Scientific associations worldwide. Citation: Tani Y, Tajima T, Kawai A, Unuma Y , Kinoshita H, et al. (2014) Evaluation of a Novel Automated Machine, the Auto2D, for Two-Dimensional Gel Electrophoresis. J Proteomics Bioinform 7: 108-111."
  • Share this page
  • Facebook
  • Twitter
  • LinkedIn
  • Google+
  • Pinterest
  • Blogger

Last date updated on April, 2021