"Protein cysteine redox status, two types of proteomic methods have been frequently used: gel-based and MS- based methods. The gel-based method has been widely applied in distinguishing the differentially post-translation modified proteins, such as redox modification of cysteines. One example is the non- reducing/reducing diagonal 2D-PAGE, which analyzes the global disulfide proteome before the thiol-reactive reagents were introduced into the study of the thiol redox status. Other improved gel-based methods are dependent on the cysteine alkylation reagent-mediated radioactive 14 -labeling, the fluorescence-labeling and the biotin- PDP-labeling, which were combined with the two-dimensional differential gel electrophoresis to develop the method of Redox-DIGE.
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Citation: Hu Q, Guo G, Yang Z, Li Y, Xia Y (2014) Stable Isotope Metabolic Labeling-Based Quantitative Thiol Redox Proteomic Analysis of Hydrogen Peroxide-treated Arabidopsis plant. J Proteomics Bioinform 7: 121-133."
Last date updated on September, 2020