Building of protein-protein interaction networks by affinity proteomic approaches can help in characterization of functional roles of proteins under experimental conditions. In an ideal condition, placing protein into an interaction network identifies a protein directly as a player in the disease process under investigation. In addition, protein-protein interaction studies can be used to shed light on mechanism other than direct inhibition or activation by which a drug can modulate target activity. Differential protein complex formation with and without compound treatment, either in cell lysate or during the purification procedure, allows the identification of compound sensitive protein-protein interaction. Vs Nitish Kumar Mishra, Application of Computational Proteomics and Lipidomics in Drug Discovery.
Last date updated on July, 2021