"Heat alone was insufficient to recover high quality protein extracts from FFPE tissue. Elevated pressure promotes water penetration into the inner core of proteins, causing denaturation, whereas heat alone causes protein unfolding followed by aggregation. Consequently, we hypothesized that the combined effects of heat and elevated pressure would facilitate the re-hydration of highly aggregated proteins in the tissue surrogate, greatly improving protein solubilization while simultaneously reversing protein-formaldehyde adducts and cross-links.
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Citation: Fowler CB, Oââ¬â¢LearyTJ, MasonJT (2014) Improving the ProteomicAnalysis of Archival Tissue by Using Pressure-Assisted Protein Extraction:A Mechanistic Approach.J Proteomics Bioinform7: 151-157."
Last date updated on September, 2024