Attempts were made to purify immunoglobulins from serum of Schizothorax, Schizothorax niger, which had been immunized with
Pomphorhynchus (Acanthocephalan) antigen. Initially, the protein in the serum was salted out at 50% saturation with ammonium sulphate and was chromatographed successively by gel filtration and ion
exchange columns. The Pomphorhynchus kashmirensis
agglutination positive fractions from ion exchange column
when concentrated and checked in SDS-PAGE, three bands where observed. Since teleosts immunoglobulins have been shown to belong to a single class, the two extra bands found in our study might be the degradation products of immunoglobulin or some unpurified contaminants. [ Shah FA, Mohanty J, Balkhi MH, Bhat FA, Chishti I
(2013) Purification of Schizothorax Immunoglobulin Raised Against Metazoan Parasite: A Preliminary Study].
Last date updated on June, 2014