"Round filter paper disks of 3cm diameter were used. For each experimental unit three filter paper discs were taken. One soaked with water, second with required oil and the third with (solvent) ethanol. All three filter paper discs were air dried at room temperature prior to exposure to ants. In experimental setup, central bottle served as experimental unit while bottles on the peripheral sides served as control units i.e. water control and solvent control. Solvent treated and water treated filter paper discs were placed in their respective side bottles. Fifteen ants were placed in the central bottle that already contained oil treated filter paper disc. All bottles were closed with lids and covered the whole experimental setup with black cloth for the maximum activity of ants.
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Citation: Mehmood F, Shahzadi P (2014) Insect Toxicity and Repellent Activity of ytochemicals from âFlea killer, Boenninghausenia albifloraâ against âBlack Garden Ant, Lasius Nigerâ of Pakistan. J Bioanal Biomed 6: 005-008."
Last date updated on July, 2014