alexa Abstract | Antibiotic Resistance, Molecular Diversity & Verotoxin Characterization of E. coli Isolated from Clinical Samples
ISSN ONLINE(2319-8753)PRINT(2347-6710)

International Journal of Innovative Research in Science, Engineering and Technology
Open Access

Like us on: https://twitter.com/ijirset_r
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access

Abstract

The objective of the present study is to determine the antibiotic resistance and analyse the genetic variation of Escherichia coli isolated from clinical samples. The clinical samples were collected from M. S. Ramaiah Teaching Hospital in Bangalore, India. The samples were collected from different patient with urinary tract infection. The isolates were cultured on selective media such as EMB agar. Determination of the physiological properties of strains was performed according to the biochemical tests recommended. The antibiotic susceptibility of the 48 E. coli samples was determined by Kirby-Bauer disc diffusion method and inhibition zone was measured. The bacteria showing zone of inhibition 10mm or less are considered as resistant bacteria and denoted as (R). For all the antibiotics these sample showed mixed results for susceptibility and resistance. Among the 48 samples 20 samples showed multiple drug resistance (MDR). After the E. coli cultures were screened for drug resistivity, their DNA was isolated from these cultures wherein thin strands of DNA were observed in each of the samples. From the RAPD PCR results obtained in this investigation, it was found that different banding patterns of the amplified products generated by 4 primers, had allowed the amplification of the DNA fragments of the E. coli isolates. The fingerprints generated by Gene i4 and Gene i6 primers revealed unique profiles for each strain in terms of number and position of bands. Subsequently, Verotoxin gene was detected using PCR with specific primers. Such information will be useful in classification, epidemiological survey, ecology and diagnosis.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Ayesha Sultana, Drona Bhattacharya, Sammya Kumar Sen, Sreyasi Bhattacharya, Lakshmi Kanth R.N.

Keywords

E. coli, Antibiotic, Drug Resistance, RAPD-PCR, Diversity, Verotoxin gene, Clinical Chemistry,Molecular Degeneration Amsler Grid

Share This Page

Additional Info

Loading
Loading Please wait..
 
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords