alexa Abstract | Anti-Cancerous Activity of Albizia Amara (Roxb.) Boivin using Human Breast Cancer Cells (MCF-7) by In Vitro Methods


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Abstract

Traditional medicinal plants in India are majorly used for treating many diseases in olden days. Based on that, the present study was aimed to investigate the anticancer effect of various extracts of leaves of Albizia amara on Human Breast Cancer Cells (MCF-7) by comparing with the 5-FU treated cells. The cytotoxicity of plant extracts were detected by MTT assay, among the three solvent extracts, the ethyl acetate extract showed effective cytotoxic action with median inhibitory concentration (IC50) of 36.31μg/ml followed by ethanol extract with 57.54μg/ml and aqueous extract with 83.18μg/ml. The ethyl acetate extract treated cells showed significant signs of apoptosis such as cell shrinkage, membrane blebbing and nuclei fragmentation. The gene expression for cancer DNA markers and Interleukins were examined by real time PCR which showed that the expression of bcl-2 was decreased in cells treated with ethyl acetate extract and with 5FU when compared with control cells. The expression of p53 and TNF-α was expressed high in cells treated with ethyl acetate extract than with control cells but was low when compared with 5FU treated cells. The expression of IL4 and IL6 was low in cells treated with ethyl acetate extract and 5FU treated cells when compared with control cells. The expression of protein such as survivin and ORP150 decreased in cells treated with ethyl acetate extract than 5FU treated cells and control cells which were detected by Bio Analyzer. These results suggest that the ethyl acetate extract of Albizia amara has potential broad spectrum of anticancer activity.

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Author(s): Gopinath P, Sundara Vadivel D, Kamatchiammal S, Saroja V.

Keywords

Albizia amara, apoptosis, cytotoxicity, ethyl acetate extract, gene expression, MCF-7, Breast Reconstruction Surgery,Cancer Screening,Exercise and Cancer,Alternative Cancer Medicines,Cancer Prevention from Nuts,Cancer Pathology,Breast Cancer Diagnosis

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