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Research Article Open Access
Lung cancer is a disease characterised by uncontrolled cell growth in tissues of the lung and is the commonest form of death worldwide due to cancer. Medicinal plants are considered to be the exclusive source of life saving drugs for majority of world’s population. The present study was carried out to evaluate the anticancer activity of various extracts of leaves of Cadaba fruticosa in human lung cancerous cell line A549 and 5-fluorouracil, by in vitro methods. Cytotoxicity of leaf extracts were assessed by MTT assay. Ethyl acetate extract was found to be more cytotoxic with a median inhibitory concentration (IC50) of 56.23 μg/ml when compared to Ethanol extract (72.44 μg/ml) and aqueous extract (81.28 μg/ml). Ethyl acetate extract treated A549 cells showed characteristics of Apoptosis like cell shrinkage, chromatin condensation and nuclei fragmentation. Expression levels of proteins were studied using bio analyser and expression of C- reactive protein and Phospho-histidine phosphatase was found to be decreased in ethyl acetate extract treated cells, when compared to control cells. Real time PCR was used to determine the gene expression levels. Expression of p53 and TNF-α was found to be high in cells treated with ethyl acetate extract than with control cells, but was low in comparison to standard 5 – Fluorouracil treated cells. Expression of Bcl-2 and IL-6 was low in cells treated with ethyl acetate extract and 5 – Fluorouracil, when compared with control cells. These results reveal the potential anticancer activity of ethyl acetate extract of Cadaba fruticosa.
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Author(s): Sundara Vadivel D, Gopinath P, Jayakar Santhosh D, Balakumaran P
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