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Research Article Open Access
In this study bioaccumulation and biodegradation of Cr(VI) and phenol was carried out using bacterium Bacillus sp. (MTCC No. 3166) and Escherichia coli (NCIM No. 5051) from single and binary substrate solution of Cr(VI) and phenol. Shake flask batch experiments were performed in an incubator cum shaker at 37ºC temperature. The bacterium Escherichia coli was acclimatized to the various concentrations of Cr(VI) and phenol in single and binary substrate solution. The binary substrate solution contains Cr(VI) and phenol in (1:2) ratio. The growth of bacterium in the presence of toxic pollutant Cr(VI) and phenol was analysed in UV spectrophotometer at 600 nm. The initial concentrations of Cr(VI) was varied from 5-25 mg/L for single substrate solution of Cr(VI) for pure culture of Escherichia coli, 10-50 mg/L for single substrate solution of Cr(VI) and 20-100 mg/L of phenol for pure and consortium culture of Escherichia coli and Bacillus sp.
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Author(s): Ankur Gupta* and Chandrajit Balomajumder
Escherichia coli, Bacillus sp., single, binary, substrate, acclimatization, bioaccumulation, biodegradation., Plant sciences, Environmental Sciences