alexa Abstract | Cloning and Expression Analysis of Vv-lac3, A Novel Functional Laccase Gene Involved in Stipe Elongation, Based on the Genomic Sequence of Volvariella volvacea
E- ISSN: 2320 - 3528
P- ISSN: 2347 - 2286

Research & Reviews: Journal of Microbiology and Biotechnology
Open Access

Like us on: https://twitter.com/BiotechMicrobio
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access

Abstract

Fungal laccases always play important roles in various physiological and developmental processes. Here, we cloned a laccase gene, and named Vv-lac3, cDNA from the Volvariella volvacea, and expressed the cDNA in Pichia pastoris. The Vv-lac3 cDNA consisted of 1599 bp containing an open reading frame (ORF) encoding a 532 amino acids. The analysis of the deduced amino acids revealed that Vv-lac3 possessed a 19-amino acids signal peptide at the N-terminal end and a 513-amino acid mature protein. The Vv-lac3 contained four conserved copper-binding sites that which is the typical structure of fungal laccases. Phylogenetic analysis showed that Vv-lac3 had a high degree of identity with other basidiomycete laccases. Native-PAGE and SDS-PAGE analysis demonstrated that the product of Vvlac3 cDNA from P. pastoris was a functional laccase with a molecular mass of 65 kDa. The expression of the Vv-lac3 gene in V. volvacea increased during button stage to the elongation stage; it reached peaked in the elongation stage, and then decreased in the maturation stage, which suggests that this gene plays a regulatory role in stipe elongation in V. volvacea.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Yuanping Lu, Guangmei Wu, Lingdan Lian, Lixian Guo, Zhiyun Yang, Wei Wang, Binzhi Chen, Baogui Xie

Keywords

Volvariella volvacea, Laccase, Fruiting body formation, Enzyme, Syndrome X,Syndrome X,Gene Expressions, Molecular and Medicine science,Venous Insufficiency, Medicinal Biotechnology, Clinical Microbiology Research, Clinical Microbiology Reviews, Clinical Microbiology and Infection, Clinical Microbiologist, Manual of Clinical Microbiology, Clinical Microbiology Case Reports, Antimicrobial Activity, Antimicrobial Agents, Antimicrobial Suceptibility,Genes and Immunity,Fermentation in Biotechnology

Share This Page

Additional Info

Loading
Loading Please wait..
 
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords