alexa Abstract | Complexity of Odontoblast and Subodontoblast Cell Layers in Rat Incisor
e-ISSN: 2320-7949 and p-ISSN: 2322-0090

Research & Reviews: Journal of Dental Sciences
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access

Abstract

Structural and functional interrelationships between odontoblast (OCL) and sub-odontoblast cell layers (SOCL) are relevant to tissue formation and regulation in health and disease, but are incompletelyunderstood. This study employed contemporary immunohistochemical techniques to gain important new insights on tissue complexity within the apical half of the rodent mandibular incisor. Whole incisors were removed from freshly killedWistar rats, fixed in 4% paraformaldehyde,demineralized, and prepared for standard immunohistochemistry. Tissues were labelled with primary antibodies to vimentin, α actin, NaKATPase, alpha tubulin and COX 1, and secondary antibodies alexafluor 488 and 594, before examination by fluorescence microscopy. Odontoblasts presented in stratified or pseudo-stratified arrangements, rather than simple columnar form. Additional stellate cells,α-actin immunoreactive, were observed predominantly in the distal half of the OCL. Cells in the cell rich zone (crz) also showed complexity, with high cell density, and cells presenting with large nuclei and sparse cytoplasm. These cells showed intense NaKATPase immunoreactivity. Some of these cells sent processes towards and into the OCL, whilst other processes remained within the crz. No processes directed downwards. The OCL of the rat mandibular incisor presents a hitherto undescribed complexity, in terms of cellular arrangement and composition, the functional significance of which is not immediately apparent. The observation that cellular processes from crz tothe OCL and lateral processes within crz suggest functional connectivityand cell to cell communication within the layer. The intense immunoreactivity to NaKATPase suggests functional specification and a high degree of tissue activity. Structural observations will require further investigations.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Alhelal A, Mahdee A, Eastham J, Whitworth J, Gillespie JI

Keywords

Odontoblast, Pulp biology, Fibroblast, Cell biology, Complexity, Cell signalling, Dental Engineering, Dental Disorders

Share This Page

Additional Info

Loading
Loading Please wait..
 
 
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords