alexa Abstract | Development before and after cryopreservation of porcine parthenogenetic embryos derived from early-delipated oocytes
E- ISSN: 2320 - 3528
P- ISSN: 2347 - 2286

Research & Reviews: Journal of Microbiology and Biotechnology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access


The present study investigated the effect of delipation (lipid droplet removal) on the developmental competence of porcine oocytes. Delipated (+/−) and/or vitrified (+/−) oocytes were subjected to parthenogenetic activation, the viability of the early parthenogenetic embryos was recorded, and the embryos were then transferred to recipients for further development. The results showed that the cleavage and blastocyst rates of the parthenogenetic embryos from the early delipated oocytes were significantly lower than those of embryos from a control group (undelipated oocytes) (P<0.05). After transfer to recipient pigs, these embryos were able to further develop and to produce parthenogenetic fetuses. Using the minimum volume cooling (MVC) method, the parthenogenetic embryos from early delipated oocytes could be cryopreserved by vitrification at 1-cell to early blastocyst stages. After thawing, the early blastocyst stage was found to be optimal for vitrification. Also embryos vitrified at 2-4 cells derived from early delipated oocytes were transferred into two recipient pigs, and resulted in limb-bud stage fetuses. In conclusion, the results demonstrated that parthenogenetic fetuses can be produced from porcine embryos before or after vitrification by a strategic combination of in vitro-matured (IVM) oocyte delipation with vitrification at any early embryonic developmental stage. This approach may have application to cryopreservation of cloned and intracytoplasmic sperm injection (ICSI) embryos derived from delipated oocytes.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Yubo Qing, Wenmin Cheng, Yingchao Liua, Xiaobing Lia, Weirong Pana, Honghui Lia, Si Lia, Baoyu Jiaa, Hongye Zhao*, Hongjiang Wei*


Porcine, Oocyte, Delipation, Cryopreservation, Parthenogenesis., Industrial Biotechnology, Microbial Cell Biology, Microbial Ecology, Microbial Fuel Cells

Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version