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Research Article Open Access
Rauwolfia serpentina commonly known as Sarpgandha is a well known medicinal plant possessing several medicinal and pharmacological activities. Extreme effectiveness of plant against snake and insect bites have made the plant popular around the globe. Over the time plant has become endangered mainly due to overexploitation and comparatively moderate propagation rate in nature. Present study reports a method of direct in vitro regeneration of shoot from in vitro cultured leaf segments of R. serpentina. About 68% cultured developed in vitro shoots from cultured leaf segments onto MS+8μM NAA+4μM BAP. Medium enriched with higher concentration of NAA was also found suitable for in vitro rooting.
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Author(s): Varnika Choudhary, Shalini Singh and Nishesh Sharma
In vitro culture, leaf, conservation, Rauwolfia, Plant sciences and Environmental Sciences