alexa Abstract | Effect of Ethyl Alcohol on the Dinitrosalicylic Acid Assay for Reducing Sugars
e-ISSN: 2319-9849

Research & Reviews: Journal of Chemistry
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Research Article Open Access

Abstract

The determination of reducing sugars, especially glucose, is of considerable interest. An assay attributed to Miller using 3,5-dinitrosalicyclic acid (DNS) is widely used but suffers from some limitations. It is, nonetheless, convenient and recommended by the IUPAC Commission on Biotechnology for measuring cellulase activity. We found that 5-10% concentrations of methanol, ethanol, 1- and 2-propanol interfered with the DNS assay and gave a positive error (an apparent increase in glucose) when aqueous standards were used for calibrating the assay. For ethanol concentrations beyond about 15%, the assay gave a negative error. In the case of ethanol, this was due to an effect of ethanol on the color-forming reaction as well as a slight effect on the spectral absorbance of the product.

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Author(s): Nyla Z. Numan and John C. Ford

Keywords

Ethyl Alcohol, Reducing sugars, Glucose, Methanol, Ethanol, 1- and 2-propanol,DNS assay, Organic chemistry

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