alexa Abstract | In vitro Antioxidant Activity of Different Extract of Callistiemon viminalis.


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Abstract

Free radicals are implicated for many diseases including diabetes mellitus, arthritis, cancer, ageing. etc. In the treatment of these diseases, antioxidant therapy has gained a great importance. Ideally an antioxidant agent should have high percentage of cure with a single therapeutic dose, free from toxicity to the host and cost effective. None of the synthetic drugs available meets there requirements. The origin of many effective drugs is found in traditional medicine and in view of this several researchers have undertaken studies to evaluate folklore medicinal plants for their proclaimed antioxidant activity. Currently much interest has been paid in the searching of medicinal plants with antioxidant activity which may lead to the discovery of new therapeutic agent that is not only used to suppress the free radicals but also used in diverse disease conditions. Different extract (water, alcoholic, chloroform and petroleum ether) of Callistiemon viminalis was studied for its in vitro antioxidant activity using Nitric Oxide Radical Inhibition Assay (NORIA). The results were analyzed statistically by the regression method. Its antioxidant activity was estimated by IC50 value. The results showed that the water and alcoholic extracts of Callistiemon viminalis showed potent antioxidant activity than the chloroform and petroleum ether extracts. The antioxidant potency of different extracts has been expressed as in order of Water>Alcohol>Chloroform>Petroleum ether. In all the testing, a significant correlation existed between concentrations of the extract and percentage inhibition of free radicals. The antioxidant property may be related to the polyphenols and flavonoids present in the extract. These results clearly indicate that Callistiemon viminalis is effective against free radical mediated disease.

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Author(s): Akhilesh Mehta, Kshitij Agarwal, Prem Saini

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