Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access


Human bone marrow derived mesenchymal stem cells (BMMSCs), a source of important autologous stem cells without ethical debate, have been used in both experimental and clinical treatment. Therefore, this research aims to isolate, culture and cryopreserve BMMSCs. Mesenchymal stem cells (MSCs) were preserved in each cryovial by slow freezing – rapid thawing method. Post-cryopreserved MSCs was analyzed the viability and the stemness by their expression of some specific markers, differentiation potential and proliferation ability. The results of this research showed it is easy to isolate the BMMSCs by culturing the mononuclear cells derived from human bone marrow in DMEM/F12 plus 15% FBS, 10 ng/ml FGF and 1% antibiotic–mycotic; the viability rates of thawed MSCs were 72.95+/-6.14%. Cryopreserved MSCs maintained the stemness after 1 year storage in nitrogen liquid. They expressed CD13, CD44, CD90, CD166 markers and were negative for CD14, CD34, CD45 and HLA-DR markers in similar to fresh MSCs. In addition, they also owned and maintained adipogenic differentiation potential. So that BMMSCs can be in vitro cultured, proliferated and preserved and used for long periods of time in regenerative medicine.

To read the full article Peer-reviewed Article PDF image

Author(s): Chung C Doan, Nhung H Truong, Ngoc B Vu, Tam T Nguyen, Hoang M Nguyen, Khue G Nguyen, Si M Do, Ngoc K Phan, Phuc V Pham


Bone marrow, Bone marrow derived mesenchymal stem cells, Mesenchymal stem cells, Stem cell cryopreservation, Bone Marrow Donation Risks,Bone Marrow Biopsy,Bone Marrow Extraction/Harvesting,Bone Marrow Transplantation

Recommended Journals

Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version