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Research Article Open Access
Recent studies indicate that microwave assisted extraction technique for extraction of constituents from plant material is good alternative to conventional extraction method. A microwave assisted extraction technique was developed to optimize the extraction and isolation of pectin from several commonly used plant sources. Aqueous extract from two plants containing pectin like Emblica officinalis and Linum unitatissimum were prepared by using conventional and microwave method. The yields obtained by microwave extraction were found to be higher than conventional extraction method. The optimization of microwave irradiation was carried out at several intensities and for different time periods. Microwave extraction at 140w intensity and 10 minute heating period produces higher yield of pectin when compared to 1hr conventional heating in both two plant sources used. The yield of pectin from Emblica officinalis and Linum unitatissimum by microwave method was found to be 8.88% and 1.49% more than obtained by conventional method, respectively at 140w intensity for 10 minute. However at higher intensity and more duration of time involve lesser yield as compared to 140w intensity and 10 minute duration of heating. This indicates that an appropriate intensity and suitable time period gives the better yield of product by microwave irradiation method. More time duration and higher intensity may also degrade the product or cause charring of the product. Product obtained by both methods was similar nature in physical and chemical properties. The developed microwave procedure can be used successfully in commercial and routine laboratory for the isolation of pectin.
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Author(s): Anil Kumar Agarwal, Pradeep Goyal, Lakashminarasimhaiah, Praveen Goyal, Prashant Desai, G. K. Singh