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Research Article Open Access
The crude extract/fractions of the leaves of Murraya exotica L. have been tested for the qualitative phytochemical analysis, quantitative estimation of total phenolic content and total flavonoid content, hydroxyl radical scavenging activity employing site-specific and non site-specific deoxyribose degradation assay and plasmid nicking assay. The study showed that flavonoids, phenols and tannins were present, but steroids, anthocyanins, proteins, carbohydrates and phlobatanins were absent in the crude extract and its different fractions. It also exhibited the presence of gallic acid, catechin, chlorogenic acid, epicatechin, caffeic acid, umbelliferone, coumaric acid, rutin, tert-Butyl hydroquinone, quercetin and kaempferol in all the fractions viz.; hexane fraction (HF), chloroform fraction (CF), ethyl acetate fraction (EAF), n-Butanol fraction (BF) and water fraction (WF) whereas ellagic acid was detected only in CF. The gallic acid was absent in CF. The investigation involving quantitative estimation of total phenolics showed the highest average of total phenolic content (226.34 μg GAE/mg) in EAF and the highest average of total flavonoids content (304 μg RE/mg) at 1000 μg/ml concentration in CF. HF showed the lowest IC50 value of 1.63 μg/ml in site-specific deoxyribose degradation assay whereas WF showed the lowest IC50 value of 0.00006 μg/ml in non site-specificdeoxyribose degradation assay. In plasmid nicking assay, the crude extract and its fractions viz. HF, CF, EAF, BF and WF exhibited hydroxyl radical scavenging activity in a dose dependent manner, with maximum activity at 1000 μg/ml, whereas EAF at 200 μg/ml exhibited the least activity.
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Author(s): Saloni Sharma, Saroj Arora