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Research Article Open Access
The aim was designed to isolate, elucidate and carry out antimicrobial activity of the isolates against bacterial and fungal strains. Using several chromatographic separation techniques, three known flavonoids, 3’,4’,7-trihydroxy- 5-methoxyflavone (5-methoxyluteolin) 1, 3’,4’,3,4,7,8-hexahydroxyflavanol (melacacidin) 2, and 3’,4’,7,8-tetrahydroxyflavanone (isookanin) 3 along with three other known constituents identified as, 2,3-epoxy-3-[4-hydroxy-3,4- dimethoxyphenyl]propan-1-ol 4, D-chiro inositol (pinitol) 5 and protocatechuic acid 6 were isolated and reported for the first time in Albizia adianthifolia. The structure of these compounds were elucidated by means of spectroscopic methods such as UV, IR, MS, 1D and 2D (COSY, HMQC, HMBC, NOESY) NMR and by comparison of their data with those of published compounds. Using TLC plate agar overlay technique, the preliminary antimicrobial activity of these compounds were tested against some selected bacterial strains E. coli, P. aeruginosa, B. subtilis, S. aureus and a fungus C. albicans and the results suggests weak to moderate activity.
Phytochemicals, Spectroscopy, Antimicrobial activity, Albizia adianthifolia, Phytochemical,Phyto Chemistry,Chemical Biology