alexa Abstract | Purification and Characterization of Manganese Peroxidase from Musa Acuminata Stem and Its Effect on Degradation of Dye Effluent
ISSN ONLINE(2319-8753)PRINT(2347-6710)

International Journal of Innovative Research in Science, Engineering and Technology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access


Various applications of Manganese Peroxidase such as transformation of dyes, biobleaching, biopulping, treating wastewater contaminated with phenols, chlorinated phenol & cresols enhances the interest for further study on the enzyme. The present aim of the study is to purify Manganese peroxidase from plant source Musa acuminata stem, an agro waste and to monitor its effect on effluent degradation. Manganese peroxidase was extracted from Musa acuminata stem juice using 0.1M Phosphate buffer, Ammonium Sulphate precipitation & Dialysis techniques were carried out to partially purify the enzyme. Column chromatography was packed with silica gel and chloroform with 0.1M Phosphate Buffer as elution buffer to achieve 10.8 fold purification with 32% enzyme yield, specific activity was found to be 24.5 U/mg. The Km and Vmax value with MnSO4 as variable substrate were calculated from MM and double reciprocal plots. The pH and temperature of the enzyme were optimized. The FTIR analysis of the degraded effluents was analyzed.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Jeniffer Sylvia D, Jhansi Lakshmi V, Renuka Devi P

Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version