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Research Article Open Access
Aim: The present contribution was aimed at the quantitative analysis of Araq-e-Bedmusk (floral aromatic water obtained from Salix caprea). Furthermore, this study was also aimed to determine its antioxidant and anti-inflammatory activities. Ethanopharmacological relevance: Unani physicians prescribe it in nervousness and palpitation, also as a supporting medicine in psychological stress and cardiac affections. Methods: The floral water (Araq-e-Bedmusk) was extracted with n-hexane and petroleum ether separately. Antioxidant activity was assessed as free radical scavenging capacity (RSC) towards 2, 2- diphenyl-1-picrylhydrazil (DPPH) radicals. Anti inflammatory activity was examined by HRBC membrane stabilization method and Quantitative analysis of the aromatic water was evaluated by GLC. Results: Quantitative analysis of petroleum ether extract of aromatic water was performed by GLC which revealed the presence of five constituents. On the other hand GLC analysis of hexane extract of aromatic water showed the presence of 7 constituents. The percentage scavenging effects of aromatic water of Salix caprea on DPPH radical were 25.50 %, 32.98 % and 39.83 % at 1 ml, 2 ml and 3 ml respectively. Furthermore, percentage stabilization of HRBC membrane of this aromatic water was 2.68, 14.23 and 35.34 at 1 ml, 2 ml and 3 ml, respectively. Conclusion: The percentage of these constituents in the aromatic water plays an important part in determining its quality. The potent antioxidant activity and significant anti-inflammatory potential of aromatic water may justify the Unani claim. It should be noted that the GLC analysis and pharmacological evaluation of the aromatic water (Araq-e-Bedmusk) from Unani Dawakhanna are the first time report from the Salix caprea.
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Author(s): Ajaz Ahmed, Wajahat A. Shah, Seema Akbar, Shamshad A. Khan