alexa Abstract | Role of in-silico COX2 molecular docking bioinformatics tool and in-vivo carrageenan induced various inflammatory phase in mouse and rat models, in evaluation of anti-inflammatory activity of molecules

Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access


In- silicomolecular docking study is used to screen new molecules for anti-inflammatory activity to evaluate their potency by in-vivo studies in animal. In the present investigation, aspirin was used as a ligand for docking with COX2 inflammatory protein by Accelrys Discovery Studio v2.1. In-vivo anti-inflammatory activity of aspirin was evaluated in C57Bl/6 mice and Wistar rats. Carrageenan was injected in intraplantar region of mice (300μg/paw) and rat (4000μg/paw). Aspirin was administered 91mg/Kg and 60mg/Kg P.O. Paw volume was measured by plethysmometer. Aspirin docked at 3rd and 4th Active Binding Site (ABS) of COX2 protein with 36.346 and 49.9 dock score with one hydrogen bond with Trp387 and four hydrogen bonds with Lys166, Lys468, and Glu465 amino acid respectively. In C57Bl/6 mice of 7 to 8 weeks old, and 30 to 35g body weight biphasic inflammatory phases (acute and chronic) were observed whereas in rat only acute phase of inflammation was observed. Aspirin could potentially inhibited late inflammatory response of 72nd h (79%) than 7th h (25.3 %) in mice. However, aspirin inhibit inflammatory phase at 7th h (62%) in rat. Results showed the correlation between in-silico and in-vivo studies. In drug development process, new molecules initially screened for molecular docking with COX2 protein, and only higher dock score molecules forward for in-vivo anti-inflammatory mice paw edema using C57Bl/6 of 7 to 8th week and 30 to 35gm weight

To read the full article Peer-reviewed Article PDF image

Author(s): Chetan Nimgulkar, Sravani Edula, Venu Racha, M. Chalamaiah, P. Shyam, A. Ravindranath, B. Dinesh Kumar

Recommended Journals

Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version