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Research Article Open Access
(E,E)-geranyllinalool (GL) can be used as an intermediate for chemical synthesis of several drugs as well as an insect pheromone and spice. Traditionally, (E)-nerolidol was used as a substrate to make (E,E)- geranyllinalool but the yield was low due to the multiple and complicated chemical synthesis processes. In this study, the (E,E)-geranyllinalool synthase gene (AtGES, At1g61120) from Arabidopsis thaliana was inserted into the Escherichia coli vector pET30a(+). E. coli strains containing plasmids pIRS and pGG that enable the synthesis of (E,E)-geranyllinalool precursors, when co-transformed with AtGES, were able to synthesize (E,E)- geranyllinalool with a yield up to 31.42 mg/L. This is the first report about the production of (E,E)-geranyllinalool by manipulation of multiple genes of its biosynthesis pathway in E. coli. Our results may facilitate metabolic engineering towards more cost-effective production of (E,E)-geranyllinalool in microorganisms.
(E,E)-Geranyllinaloolsynthase, E. coli expression, Metabolic engineering, GC-MS, Microbiology, Biotechnology, Advances in Food Bioprocess Technology, Bioprocess Industry and Market Analysis, Advances in Biotechniques, Industrial Bioprocessing, Bioprocess and Systems Engineering, Pharmaceutical Bioprocessing, Advances in Bioprocess Technology, Advanced Bioprocess Products