alexa Abstract | Supercritical CO2 Extraction of Active Components of Fenugreek (Trigonella foenum-graecum L.) Seed


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Abstract

Use of supercritical fluids for the separation of analytes from the matrix of many samples has been of interest to researchers and industries now a day. This is because of the fact that, use of reagents of this type avoids many of the problems such as high solvent cost, environmental pollution, spent solvent impurity in the extract, longer duration of extraction etc. lying with conventional methods of extraction for organic liquid extractants. In this context, supercritical CO2 extraction (SC-CO2) of fenugreek (Trigonella foenum-graecum L.) seed was carried out in a bench scale plant. To investigate the effects of pressure and temperature on the solubility of oil and oil yield, and to optimize the operating conditions, three isobaric (100, 200, and 300 bar) and three isothermal (40, 50 and 60°C) extraction conditions were selected. The optimum extraction condition was 40°C /300 bar, and 85 min extraction time, where 3.65% oil yield was achieved. Chemical composition of the oil was investigated using gas chromatography–mass spectrometry in electron impact mode. Thirty numbers of different compounds are detected out of which 15 were fatty acids (58.44%). The major compounds are oleic acid (32.74%), heneicosane (9.50%) and sotolon (8.52%) and also contain some amount of Eucalyptol (0.85%).

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Author(s): Debashis Das, Achyut K. Panda

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