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Research Article Open Access
Rice (Oryza sativa L.) is one of the most important food crops and a primary source of food for more than half the world’s population. Rice seedlings are particularly sensitive to chilling in early spring in temperate and subtropical zones and in high elevation areas. Cold stress during rabi season at early stage of crop season cause various seedling injuries, delayed heading and yield reduction. Improvement of low temperature (cold) tolerance significantly increases rice production. In the present study 36 diverse and adopted rice genotypes were used to identify seedling cold tolerant varieties and these were genotyped with 32 Rice Microsatellite (RM) markers to identify molecular markers linked to seedling cold tolerance. All 36 genotypes subjected to cold temperatures at seedling stage both in field and at lab. Morpho-physiological traits such as leaf yellowing and SPAD chlorophyll meter readings (SCMR) identified as selection criteria for cold tolerance. SCMR were recorded before and after cold treatment which measures relative chlorophyll content of leaf. Percent decrease in SCMR was considered to identify susceptible and tolerant genotypes. Five genotypes viz., CT376, JGL18270, Chakiya Mao, JGL11470 and JGL1798 showed highest leaf yellowing score and percent decrease in SCMR after cold treatment, hence these are considered as cold susceptible genotypes. Nine genotypes (JGL17004, JGL20171, JGL19607, Tellahamsa, WGL283, BPT5204, IR83222-85, IR83222-174 and CT37) found to be tolerant to cold as they recorded lowest leaf yellowing score and percent decrease in SCMR after cold treatment. Among 32 markers one marker (RM85) showed specific banding pattern in cold susceptible and tolerant genotypes. Cosegregation of this marker will be confirmed using immortal mapping population (RILs: Recombinant Inbred Lines), which is being developed. Cold tolerant linked markers will be more useful in marker assisted pyramiding of biotic and abiotic resistance genes in rice.
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Author(s): S. Thippeswamy*, Y. Chandramohan, K. Pravalika, B. Madhavilatha and Zameema Samreen
Rice, Seedling, Tagging, Molecular Markers, Plant Sciences, Environmental sciences