alexa Abstract | To Study the Effect of Activated Charcoal, Ascorbic Acid and Light Duration on InVitro Micropropagation of Aloe vera L
ISSN ONLINE(2319-8753)PRINT(2347-6710)

International Journal of Innovative Research in Science, Engineering and Technology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access


The research was conducted to determine the effect of ascorbic acid (50 mgl-1, 100 mgl-1, 200 mgl-1) and activated charcoal (0.5 gl-1, 1 gl-1, 2 gl-1) independently with different light duration (darkness for 4 weeks, and 16 hours light for 4 weeks followed by 2 weeks in 16 hours light) on shoot regeneration. Explants of Aloe vera were planted on MS basal media supplemented with 1.6 mgl-1 IAA, 4.0 mgl-1 BAP and cultured for 4 weeks. After 4 weeks, degree of explant browning was evaluated. Explants were then cut vertically into two pieces and planted on shoot regeneration media. After 4 weeks more number of shoot developed on shoot regeneration medium, i.e. MS medium supplemented with 1.6 mg/l IAA + 4.0 mg/l BAP + 100 mg/l ascorbic acid + 0.5g/l activated charcoal. Browning of explants was minimized in this medium and elongation of micro-shoots and the growth of the plantlets were also better. Further elongation and rooting of micro-shoots were obtained when sub-cultured on to MS basal medium containing 0.5g/l activated charcoal and 100% of the survival of rooted plantlets was observed after acclimatization. Therefore, the above protocol could be effectively used in rapid mi- cropropagation of elite plants of Aloe vera.

To read the full article Peer-reviewed Article PDF image

Author(s): Priyanka Das , Alok Kumar Srivastav

Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version