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Research Article Open Access
Seasonal variation imparts environmental stress in dairy animals, and impairs animal health and productivity. In order to measure the performance ability of a particular breed/animal towards heat stress, it is imperative to determine its thermotolerance ability. Heat shock proteins (HSPs) are molecular chaperons that maintain native conformation of proteins and cell viability during stress period. The peripheral blood mononuclear cells (PBMCs) have been widely utilized as a cellular model to understand the differential heat shock response across livestock species. The present investigation was attempted with the assumption that seasonal variation can lead to changes in the level of heat shock protein transcripts in dairy breeds. Therefore, this study aimed at assessing the transcriptional stability of heat shock protein family genes and cell proliferation rate in Sahiwal cows (Bos indicus), Holstein cows (Bos taurus) and Murrah buffaloes (Bubalus bubalis). The basal expression level of heat shock protein family genes (HSP27, HSP40, HSP60, HSP70 and HSP90) were analysed in PBMCs during peak summer and winter seasons. In comparison to Sahiwal cows and Murrah buffaloes, relative expression data showed significant induction of HSP70, HSP40, HSP60 and HSP90 mRNA in HF cows during summer stress. The cell proliferation data further indicated significant (p<0.05) inhibition of cell proliferation in Holstein cows during summer stress. Overall data on transcriptional stability of HSPs mRNA and cell proliferation suggested better survivability of Sahiwal cows PBMCs to summer stress in comparison to HF cows.
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Author(s): Amit Kishore, Monika Sodhi, Ankita Sharma, Umesh K Shandilya, Ashok Mohanty, Preeti Verma, Sandeep Mann, Manishi Mukesh