alexa Abstract | Validation of Spore-Forming Organisms Recovery from Peroxygen-Based Disinfectants


Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access

Abstract

Neutralization method evaluation is a crucial preliminary step in biocidal agent evaluation and environmental monitoring (EM) programs. In the present study, four commercial peroxygen/Ag+-based sporicidal disinfectants in pharmaceutical industry were tested against 3 spore-forming microorganisms (Aspergillus brasiliensis, Bacillus subtilis subsp. spizizenii and Bacillus cereus). An in-house made neutralizing broth was used to dilute biocidal agents to 1:10 and 1:100 (v/v) dilutions. Disinfectant preparation, dilution and all other test conditions were performed under the laboratory conditions that simulated the working environment. Each of the neutralizer toxicity and efficiency was examined using the statistical comparison among three experimental groups viz: viability control, neutralizer toxicity and neutralizer efficacy. Different analysis criteria were investigated to compare the rate of success and failure of each replicate group for neutralizer efficacy (NE) and neutralizer toxicity (NT). Criteria of comparison were Harmonized Chapter USP<61>, USP<1227> of 3 independent replicates recovery in agar medium and finally Sutton initial recovery criteria of ≥0.75 was applied followed by Dunnett's Multiple Comparison Test in case of suspect failure. These criteria were in agreement; where Bacillus cereus gave the highest rate of failure in neutralization study. Disinfectants Mil and Pury were the hardest to neutralize and Bacillus species were sensitive to them in the neutralization procedure. Bixco disinfectant was the most successfully neutralized at 1:10 dilution with the 3 organisms. The in-house made chemical neutralizer was effective in microbial spores' recovery from residual sporicidal biocides at 1:100. Thus, it is suitable to be incorporated in both EM media and sanitization program validation.

To read the full article Peer-reviewed Article PDF image

Author(s): M. E. Eissa, A. S. Nouby

Keywords

Aspergillus brasiliensis, Bacillus species, in house made neutralizing broth, neutralizer efficacy, neutralizer toxicity, peroxygen/Ag+- based sporicidal, Transmission of Diseases from Mother to Child

Recommended Journals

Share This Page

Additional Info

Loading
Loading Please wait..
 
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords