"Serial dilution agar plating method was employed for the isolation of soil microbes; the suspension was diluted up to 10-5. The aliquots were cultured for fungus on Czapek Dox Agar (NaNO 32.0 g, KCl 0.5 g, K2HPO4 1.0 g, MgSO 4 .7 H2O 0.5 g, FeSO4.7H 2 O 0.01 g,); and Potato Dextrose Agar (Peeled potato - 200.0 g, Dextrose - 20.0g) media. For primary isolation, Rose Bengal (30mg/L) was also added to the medium. Three plates were incubated for 24-96 hrs at 25 Â± 2Â°C, and morphologically unique fungi were sub-cultured and purified using standard techniques.
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Last date updated on July, 2014