Spectrofluorometer

"The relative fluorescence intensity was measured in the germinating spores (104 spores/ml) of the transformed isolates using spectrofluorometer at 460-500 nm excitation wavelength and emission wavelength of 510-560 nm. For detection of GFP fluorescence by using confocal microscopy, the small amount of untransformed control and GFP transformed fungal tissue were taken separately on a glass slidein a drop of water. After placing the cover slip, the slides were sealed properly and the fluorescence was visualized under the microscope using GFP filters (excitation- 489 nm and emission-511 nm). OMICS Group International is one of the leading Open Access Publishers which is publishing 700+ peer-reviewed journals with the support of 50,000+ editorial board members as editorial team and aimed to disseminate the scholarly knowledge to the scientific society. OMICS Group also organizing 3000+ International Scientific Conferences and events yearly all over the world with the support of 1000+ Scientific associations worldwide.

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Last date updated on September, 2024