"The relative fluorescence intensity was measured in the germinating spores (104 spores/ml) of the transformed isolates using spectrofluorometer at 460-500 nm excitation wavelength and emission wavelength of 510-560 nm. For detection of GFP fluorescence by using confocal microscopy, the small amount of untransformed control and GFP transformed fungal tissue were taken separately on a glass slidein a drop of water. After placing the cover slip, the slides were sealed properly and the fluorescence was visualized under the microscope using GFP filters (excitation- 489 nm and emission-511 nm).
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Last date updated on July, 2014