"In each of four 30 Ã 30 Ã 30 cm bed net netting cages, were placed 50 males and 50 females (none blood fed). Mosquitoes were 5â7 days old. With a hand sprayer, we applied the product through the mesh of mosquito bed netinto the cage to reach the mosquito adults. In each cage one of four treatments was applied: 20 mL of the oil formulation with a dose of 6 Ã 10 7 spores/mL (1 . 3 Ã 10 10 spores/m 2); for the Suneem oil control group, we applied 20mL of a solution of 20 mL Suneem diluted with distilled water to 500 mL; 20 mL of aqueous formulation at 6 Ã0 7 spores/mL (1 . 3 Ã 10 10 spores/m 2); aqueous control treated with 20 mL of distilled water only. The conditions were 25 Â± 1 Â°C and 75 Â± 2% relative humidity (RH).
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Last date updated on November, 2020