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Stable Isotope

"The stable isotope labeling into MS-based proteomics study renders it a robust and accurate approach in characterization and quantification of various PTMs in numerous organisms, it therefore becomes possible to integrate the stable isotope-based chemical labeling into thiol redox proteomics. To do that, a stable isotopic tag is often linked to protein thiol moiety is a chemical (or enzymatic) reaction during protein sample preparations. A characteristic example of application of this approach in redox proteomics is the stable isotope-coded affinity tag (ICAT)-based redox proteomics, in which a 12 C-coded and 13 C-coded 9-carbon linker was introduced and applied to quantify the redox status in two different samples. OMICS Group International is one of the leading Open Access Publishers which is publishing 700+ peer-reviewed journals with the support of 50,000+ editorial board members as editorial team and aimed to disseminate the scholarly knowledge to the scientific society. OMICS Group also organizing 3000+ International Scientific Conferences and events yearly all over the world with the support of 1000+ Scientific associations worldwide. Citation: Hu Q, Guo G, Yang Z, Li Y, Xia Y (2014) Stable Isotope Metabolic Labeling-Based Quantitative Thiol Redox Proteomic Analysis of Hydrogen Peroxide-treated Arabidopsis plant. J Proteomics Bioinform 7: 121-133."
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Last date updated on September, 2024

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