alexa Transformation Of F. Oxysporum|Journals Open Access|Transformation Of F. Oxysporum

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Transformation Of F. Oxysporum

"Transformation of F. oxysporum was done with pCAMBIA 1302 vector harboring sGFP gene under the control of CaMV35S promoter and hygromycin B phosphotransferase (HPT) as the selection markerene (generously provided by Dr. Richard A. Jefferson, CAMBIA, Canberra, Australia). Two different protocols were followed- a) F. oxysporum spores were germinated in carboxy methyl cellulose (CMC) broth (15 g carboxymethyl cellulose, 1 g yeast extract, 0.5 gMgSO4, 1 g NH4NO3 and 1 g KH2PO4, for 1 litre broth) at 100 rpm, at28ºC for 1 week. The culture was filtered through two layers of cheesecloth and spores were harvested at 4,500 rpm, 4ºC for 5 min. Usinghaemocytometer, spores were counted and diluted to 106 spores/ml ofspore suspension. Agrobacterium-mediated transformation (AMT) method and, b)glass-bead method. OMICS Group International is one of the leading Open Access Publishers which is publishing 350 scholarly open access journals with the support of 30,000 well-versed scholars as editorial team and aimed to disseminate the scholarly knowledge to the scientific society. OMICS Group also organizing 100 international scientific conferences and events yearly all over the world with the support of 100+ scientific associations worldwide.
 
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