|"Decrease in pyrene concentration to 25 ppm led to a two-fold decrease in biomass level (40% decrease,); this suggestedthat a greater sensitivity to pyrene than phenanthrene at a common low concentration (10 ppm) does not necessarily result in a proportionatelyhigher toxicity at elevated concentrations (such as 25 ppm). A completely different pattern was observed in case of benzo(a) pyrene (Figure 2C) in comparison to phenanthrene and pyrene. At 10 ppm concentration, there was a significant increase (20%) in biomassproductionin comparison to the control probably because of the recognized cell proliferation effect of this PAH toward eukaryotic cells. At 25 ppm concentration, there was a growth inhibition but the mycelial mass was reduced by only 30%, a figure significantlylower than those observed for phenanthrene (50%) and pyrene (40%). Taken together (10 and 25 ppm), fungal growth inhibition effect exhibited by the PAHs followed the trend pyrene>phenanthrene>benzo(a)pyrene, suggesting that there was no uniform pattern of tolerance across the PAH types. Since pyrene showed the lowest threshold limit of tolerance (10 ppm), it was used as a model PAH for further investigationsin mixture studies with Cd.
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