DNA Vaccination is a strategy for protecting an organism against disease by infusing it with genetically engineered DNA to release an immunological reaction. Nucleic acid immunizations are still under testing, and have been connected to various viral, bacterial and parasitic models of illness, and in addition to a few tumor models. DNA antibodies have various advantages over conventional immunizations, including the capacity to impel a wide range of immune response types.
Advantages of DNA vaccination are with no risk for infection, Antigen presentation by both MHC class I and class II molecules, Simplicity of development and production, Stability of vaccine for storage and delivery, Cost-effectiveness, Long-term persistence of immunogen, etc. Disadvantages of DNA vaccination are Risk of affecting genes controlling cell growth, Possibility of inducing antibody production against DNA, etc.
Plasmid vectors are used in DNA Vaccination. Parenteral, Topical application, Cytofectin-mediated methods are Plasmid DNA delivery methods. Formulation of DNA for parenteral method is aqueous solution in saline, DNA-coated gold beads, aqueous solution. Formulation of DNA for Topical application is aqueous solution. Formulation of DNA for Cytofectin-mediated are Liposomes, microspheres, recombinant adenovirus vectors, attenuated Shigella vector, aerosolised cationic lipid formulations.
Last date updated on June, 2014