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The technique of liquid chromatography with tandem mass spectrometry (LC-MS/MS) undertakes the indispensable role in clinical laboratory because of its unparalleled advantages of specificity, sensitivity, and multiple analytes measurement in highly complex biofluids over other techniques such as immunoassays. In the past two decades, with the continuous improvement in performance, LCMS/ MS has obtained considerable development in clinical laboratory. Currently its application area expanded to endocrinology, screening for inborn errors of metabolism, therapeutic drug monitoring/toxicology confirmation, vitamin analysis, and more recently, the peptide and protein quantitation.
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