Human cultured female osteoblasts (Obs) respond age-dependently to estradiol-17β (E2) and to phytoestrogens by increased DNA synthesis (DNA) and creatine kinase specific activity (CK). ERα mRNA expression is higher than ERβ mRNA in Obs. Pre-menopausal Ob (prOb) reveals higher expression of ERα than post-menopausal Ob (poOb), but similar intracellular and membranal E2 bindings. ERα mRNA is stimulated in prOb and inhibited in poOb by different estrogens.
Citation: Somjen D, Katzburg S, Kaye AM, Kohen F, Posner GH (2011) Age- Dependent Responsiveness of Human Female Cultured Bone Cells to Estrogenic Compounds. Endocrinol Metabol Syndrome S1:001. doi: 10.4172/2161-1017.S1-001