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Characterizing the expression of a transgene is a crucial step in all transgenic projects. The choice of whether to analyze the expression of mRNA, the protein product, or both, depends upon the specific transgene and its expected function. During the design of the transgene, one must keep in mind the strategy to be used for its detection and whether founder animals or their offspring will be used in experimentation. In analyzing transgene protein expression in cells, tissues or whole animals, a variety of immunological and non-immunological techniques, including the use of reporter genes, can be employed.
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