In Genetics and Molecular Biology, upstream and downstream both allude to a relative position in DNA or RNA. Every strand of DNA or RNA has a 5' end and a 3' end, so named for the carbons on the deoxyribose (or ribose) ring. By tradition, upstream and downstream identify with the 5' to 3' course in which RNA interpretation takes place.Upstream is toward the 5' end of the RNA particle and downstream is toward the 3' end. At the point when considering twofold stranded DNA, upstream is toward the 5' end of the coding strand for the quality being referred to and downstream is toward the 3' end. Because of the opposition to parallel nature of DNA, this implies the 3' end of the layout strand is upstream of the quality and the 5' end is downstream.