alexa Transcriptomics

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The recent RNA sequencing or RNA-Seq, involves highthroughput sequencing of short cDNA fragments obtained from the pool of RNA (total or fractionated, such as poly (A)+ or ribosomal rna depleted) to provide single-base resolution to the transcriptome. The traditional expression analysis primarily addressed the identification of differentially expressed transcripts with respect to measured variables of interest, such as differing environments, treatments, phenotypes, or clinical outcomes. The advent of RNA-Seq has provided a broad spectrum of applications and enabled researchers to address a wider range of biological problems. This technology enables cataloguing all species of transcript, including coding and non-coding mRNAs; to determine the transcriptional structure of genes, splicing patterns and other post-transcriptional modifications.

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